Thermo Fisher Scientific NFYB Monoclonal Antibody (2H2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2H2

Immunogen

NFYB (AAH05317, 1 a.a. approximately 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFYB,uniProtId:P25208-1,ncbiNodeId:9606,antigenRange:1-207,antigenLength:207,antigenImageFileName:H00004801-M08A_NFYB_P25208-1_House_mouse.svg,antigenImageFileNamePDP:H00004801-M08A_NFYB_P25208-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Storage buffer

ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE SFREQDIYLP IANVARIMKN AIPQTGKIAK DAKECVQECV SEFISFITSE ASERCHQEKR KTINGEDILF AMSTLGFDSY VEPLKLYLQK FREAMKGEKG IGGAVTATDG LSEELTEEAF TNQLPAGLIT TDGQQQNVMV YTTSYQQISG VQQIQFS

Target Information

The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold handshake motif in a manner analogous to the histone proteins H2B and H2A, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.