
Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Secondary Antibody, HRP
HRP로 결합된 Donkey anti-Goat IgG (H+L) 폴리클로날 2차 항체로, Western blot, IHC, ELISA 등 다양한 응용에 적합합니다. 고순도 친화 크로마토그래피 정제, 높은 특이성과 감도를 제공하며, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534673 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Conjugate Formats
- AP
- Biotin
- FITC
- PE
- Qdot 565
- Qdot 625
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is verified by immunoelectrophoresis (IEP).
Rehydrate with 1.1 mL deionized water, stand 30 minutes at room temperature to dissolve (overfilled to ensure complete recovery). Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins.
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins.
They are useful for detection, sorting, or purification of goat antibodies.
Secondary antibodies provide versatility and enhanced sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically produced by immunizing the host with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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