Thermo Fisher Scientific CD148 Polyclonal Antibody
Western blot과 Immunoprecipitation에 적합한 CD148 폴리클로날 항체. Human, Mouse, Rat 반응성. Goat 유래 IgG, 항원 친화 크로마토그래피로 정제. 동결건조 형태로 제공되며, -20°C에서 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunoprecipitation (IP) | 2.5 µg/500 µg cell lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human DEP-1/CD148 (Arg997–Ala1337) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607994 |
Product Specific Information
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CD148 is a highly glycosylated transmembrane protein of up to 250 kDa, containing eight fibronectin type III domains in the extracellular region and one tyrosine phosphatase domain in the cytoplasmic region. Expression is found broadly on fibroblasts, epithelial cells, lymphocytes (B, T and NK cells), and myeloid cells (monocytes and granulocytes). CD148 can dephosphorylate proteins such as LAT and PLC-gamma, and is involved in the downregulation of T cell activation. Conversely, CD148 expression can be upregulated following T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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