
Thermo Fisher Scientific Phospho-SGK1 (Ser422) Polyclonal Antibody
SGK1 단백질의 Ser422 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제 및 안정적 보관용 완충액 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of Ser422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SGK (SGK1) is a serine/threonine kinase that may be a transcriptional target for p38/MAPK in hepatoma cells. It plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential role in nephropathy and cardiovascular development.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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