Thermo Fisher Scientific Isocitrate dehydrogenase Polyclonal Antibody, Biotin
Isocitrate dehydrogenase 단백질을 인식하는 Biotin 결합 폴리클로날 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. 인간, 마우스, 랫드 등 반응성. 친화 크로마토그래피로 정제된 고품질 항체로 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–150 of IDH2 (UniProt ID: P48735-1) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one utilizing NAD and the other NADP as electron acceptors. Five isocitrate dehydrogenases have been reported: three NAD-dependent forms localized to the mitochondrial matrix, and two NADP-dependent forms, one mitochondrial and one cytosolic. The NADP-dependent mitochondrial isozyme encoded by this gene plays a key role in intermediary metabolism and energy production and may interact with the pyruvate dehydrogenase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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