
Thermo Fisher Scientific Human IFN-beta Recombinant Protein, PeproTech
인간 IFN-beta 재조합 단백질로, CHO 세포에서 발현되며 ≥95% 순도를 보장합니다. 세포독성 분석으로 활성을 검증했으며, 항바이러스·면역반응 연구에 적합합니다. 동결건조 형태로 제공되며, -20°C에서 보관합니다.
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Applications
Western Blot (WB)
- Tested Dilution: -
- Publications: 1
ELISA
- Tested Dilution: -
- Publications: 2
Functional Assay
- Tested Dilution: Assay-dependent
- Publications: 1
Inhibition Assays (IA)
- Tested Dilution: -
- Publications: 1
In vitro Assay (IV)
- Tested Dilution: -
- Publications: 92
Miscellaneous PubMed (Misc)
- Tested Dilution: -
- Publications: 18
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human, Mouse, Pig |
| Expression System | CHO cells |
| Amino Acid Sequence | MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN |
| Molecular Weight | 20 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | <1 EU/µg |
| Activity | Determined by a cytotoxicity assay using human TF-1 cells. Expected ED50 ≤ 0.1 ng/ml, corresponding to ≥ 1 × 10⁷ units/mg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
- Catalog No. 300-02BC-1MG is supplied as 2 × 500 µg (300-02BC-500UG).
- Recombinant Human IFN-beta is a 20.0 kDa protein containing 166 amino acid residues.
- Due to glycosylation, the approximate MW is 22.3 kDa (based on SDS-PAGE and Mass Spectrometry).
- Catalog Number 300-02BC replaces Human IFN-beta (Catalog Number 300-02B).
- Shipped at ambient temperature. Refer to the lot-specific Certificate of Analysis for storage, handling, and reconstitution details.
Target Information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit tumor development. IFN-beta binding activates tyrosine kinases Jak1 and Tyk2, leading to phosphorylation of STAT transcription factors and expression of immune response genes. Members of the toll-like receptor (TLR) family also stimulate IFN-beta production. Clinically, IFN-beta is used in treatments for tumors, infections, and multiple sclerosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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