Thermo Fisher Scientific Human IFN-gamma Recombinant Protein, PeproTech
인간 IFN-γ 재조합 단백질로 면역세포 활성화 연구에 적합합니다. E. coli에서 발현된 고순도(≥98%) 단백질이며, 세포독성 분석 기준 높은 생물학적 활성(≥2×10⁷ units/mg)을 보유합니다. ELISA, WB, Flow Cytometry 등 다양한 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Assay-dependent
Flow Cytometry (Flow)
- Assay-dependent
ELISA
- Assay-dependent
Functional Assay
- Assay-dependent
Inhibition Assays (IA)
- Assay-dependent
T-Cell Activation (TCA)
- Assay-dependent
In vitro Assay (IV)
- Assay-dependent
Miscellaneous PubMed (Misc)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Bacteria, Fish, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat, Virus |
| Expression System | E. coli |
| Amino Acid Sequence | MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ |
| Molecular Weight | 16.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% (SDS-PAGE gel and HPLC analyses) |
| Endotoxin Concentration | <1 EU/µg |
| Activity | ED50 ≤ 0.05 ng/ml (HT-29 cells), ≥ 2×10⁷ units/mg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human IFN-gamma is a 16.8 kDa protein containing 144 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please refer to the lot-specific Certificate of Analysis.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin, and other cytokines.
It is a dimeric protein consisting of two 146 amino acid subunits and functions as a homodimer (~45 kDa). On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Biological activity is highly species-specific; human IFN gamma does not cross-react with mouse IFN gamma.
Functions include:
- Antiviral and tumor antiproliferative activity
- Induction of class I and II MHC
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
- Cytokine regulation and synergistic action with other cytokines
Activation occurs via IFN gamma receptor I and II, triggering the JAK-STAT pathway.
Human IFN gamma shares ~40% sequence homology with mouse IFN gamma and is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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