Thermo Fisher Scientific Cox-2 Polyclonal Antibody
Cox-2 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. Western blot, IHC, ELISA, IP 등에 사용 가능하며, 액상 형태로 제공됩니다. -20°C 보관이 필요하며, 연구용으로만 사용됩니다.
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- 100-401-226
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:5,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human Cox-2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 55 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antiserum is directed against human Cox-2. Two different isoforms of the enzyme are known, Cox-1 and Cox-2. A BLAST analysis suggests cross-reactivity with Cox-2 in human, mouse, and rat based on homology with the immunizing sequence, and it should not cross-react with Cox-1. Reactivity with Cox-2 from other species is unknown.
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis, including inflammatory prostaglandins.
The conversion involves two steps:
- Cyclooxygenase (COX) reaction converting arachidonate to prostaglandin G2 (PGG2)
- Peroxidase reaction reducing PGG2 to PGH2
COX2 is constitutively expressed in tissues such as endothelium, kidney, and brain, and is up-regulated in pathological conditions like cancer and inflammation.
Up-regulation is associated with increased cell adhesion, resistance to apoptosis, and tumor angiogenesis.
COX2 is inhibited by calcitriol (active Vitamin D) and targeted by NSAIDs such as aspirin, which reduce inflammation-related pain and swelling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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