Thermo Fisher Scientific CD5 Monoclonal Antibody (YKIX322.3), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Dog

Published species

Dog

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YKIX322.3

Conjugate

PE PE PE

View additional formats

• APC-eFluor 780
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717676

Product Specific Information

Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1. CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells. Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels. In dogs, CD5 is not detectable on B cells or B cell lines.

Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YKIX322.3 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter`s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.