Thermo Fisher Scientific TCP1 Polyclonal Antibody
Thermo Fisher Scientific의 TCP1 Polyclonal Antibody는 인간, 마우스, 랫트에 반응하는 Rabbit IgG 항체로, Western blot, IHC, ELISA 등에 적합합니다. 액상 형태로 제공되며, Affinity Chromatography로 정제되어 높은 특이성과 안정성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 307–556 of human TCP1 (NP_1103792) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805407 |
Product Specific Information
- Immunogen sequence: AVRRVLKRDL KRIAKASGAT ILSTLANLEG EETFEAAMLG QAEEVVQERI CDDELILIKN TKARTSASII LRGANDFMCD EMERSLHDAL CVVKRVLESK SVVPGGGAVE AALSIYLENY ATSMGSREQL AIAEFARSLL VIPNTLAVNA AQDSTDLVAK LRAFHNEAQV NPERKNLKWI GLDLSNGKPR DNKQAGVFEP TIVKVKSLKF ATEAAITILR IDDLIKLHPE SKDDKHGSYE DAVHSGALND
- Positive Samples: U-251MG, 293T, PC-3, Jurkat
- Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly, sharing characteristics with Heat Shock Protein 70 (HSP70) such as high abundance, induction by environmental stress, and ATPase activity.
The chaperonin family includes mitochondrial HSP60, E. coli GroEL, plastid Rubisco-subunit binding protein, and archaebacterial TF55. TCP-1 shares approximately 40% identity with TF55 but minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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