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Norgen Urine Exosome Purification and RNA Isolation Maxi Kit
SKU: 58800
All-in-one system for purification of exosomes and subsequent isolation of RNA from urine samples.
Features and Benefits
- Purification and enrichment of intact urinary exosomes for functional studies
- Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
- Versatile urine input volumes
- No phenol extractions, Proteinase K treatment, or carrier RNA required
- No ultracentrifugation, filtration, or special syringes needed
- No precipitation reagents or overnight incubation required
- Compatible with urine from most species
- Purification based on spin column chromatography using Norgen’s proprietary resin
- Purified RNA suitable for downstream applications such as Small RNA Sequencing, RT-qPCR, Northern blotting, and expression assays
Kit Specifications
| Specification | Description |
|---|---|
| Minimum Urine Input | 11 mL |
| Maximum Urine Input | 30 mL |
| Size of Exosomes Purified | 40 nm – 150 nm |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50–100 μL |
| Time to Complete 10 Purifications | 35–40 minutes |
| Average Yields* | Variable depending on specimen |
*Refer to product insert (page 4) for average yields and RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be tightly sealed and stored at room temperature.
Stable for 2 years after shipment.
If salt precipitation is observed, warm Lysis Buffer A for 20 minutes at 60°C.
Important Note
Urine samples stored at -70°C, -20°C, or 4°C may develop precipitates due to protein aggregation.
Removing these precipitates can cause exosome loss and affect nucleic acid quality.
Use Norgen’s Urine Preservative to maintain nucleic acid and protein integrity for over 2 years at room temperature.
Kit Components
| Component | Cat. 58400 (Mini, 50 preps) | Cat. 58700 (Midi, 25 preps) | Cat. 58800 (Maxi, 15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 8 mL | 30 mL | 50 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Lysis Buffer A | 20 mL | 20 mL | 20 mL |
| Lysis Additive B | 2 mL | 2 mL | 2 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Mini Filter Spin Columns | 50 | 25 | 15 |
| Mini Spin Columns | 50 | 25 | 15 |
| Collection Tubes | 50 | 25 | 15 |
| Elution Tubes (1.7 mL) | 100 | 50 | 30 |
| Product Insert | 1 | 1 | 1 |
Supporting Data
Figure 5. Isolation of RNA from exosomes purified from different urine volumes
Norgen’s Urine Exosome Purification and RNA Isolation Maxi Kit (Cat# 58800) was used to isolate RNA from exosomes purified from different urine volumes.
2 μL of isolated RNA was used in RT-qPCR to assess amplification of urinary exosomal miR-30a.
The average Ct value decreased linearly with increased input volume, showing excellent linearity and >90% recovery.
Panel A – Avg. Ct Value of Urinary Exosomal miR-30a
| Urine Volume (mL) | Avg. Ct Value |
|---|---|
| 11 mL | 21.84 |
| 30 mL | 20.37 |
Panel B – Relative Amount of Urinary Exosomal miR-30a
| Urine Volume (mL) | Relative Amount | % of Linearity |
|---|---|---|
| 11 mL | 2.67 | 100 |
| 30 mL | 7.41 | 93 |
Documentation
Protocols
Safety Data Sheets
Flyers
Citations
| Title | Journal | Authors |
|---|---|---|
| A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants | Sports, 2021 | Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami, Kazuhiro Takekoshi |
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