Thermo Fisher Scientific PKC gamma Monoclonal Antibody (PKC66)
PKC gamma 단백질을 특이적으로 인식하는 단클론 항체로, Western blot 및 IHC 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트에 반응하며 뇌 및 신경계 연구에 유용합니다. 액상 형태로 제공되며 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–5 µg/mL | View 7 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| in situ PLA (PLA) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Guinea pig, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PKC66 |
| Immunogen | Purified rat brain PKC |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533015 |
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified, differing in structure, distribution, localization, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family, which are Ca²⁺- and phospholipid-dependent.
PKC family members phosphorylate diverse protein targets and play roles in various signaling pathways. They also act as receptors for phorbol esters, a class of tumor promoters. PKC gamma is expressed exclusively in the brain and spinal cord, localized to neurons, and is essential for neuronal functions such as long-term potentiation (LTP) and long-term depression (LTD). Knockout studies suggest involvement in neuropathic pain development. Mutations in PKC gamma have been linked to spinocerebellar ataxia-14 (SCA14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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