Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue/Red/Violet/Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It functions as a tyrosine phosphatase and plays an important role in lymphocyte activation.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of mouse bone marrow cells
• Recommended use ≤ 0.5 µg/test (in 100 µL final volume)
• Optimize antibody concentration empirically

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is intended for cytometers with an ultraviolet (355 nm) laser. Verify instrument compatibility before use.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation/Emission: 350 nm / 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of surface proteins in lymphocytes). It is absent on non-hematopoietic cells and tissues. CD45 regulates Src-family kinases, T- and B-cell receptor signaling, and cytokine receptor signaling. It plays roles in lymphocyte development, apoptosis, and integrin-mediated signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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