Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 661, 737, 805
• Brilliant Violet 421, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage and activated mature T and B lymphocytes.
CD25 binds IL-2 with low affinity alone, but associates with CD122 (IL-2Rβ) and CD132 (γ chain) to form the high-affinity IL-2 receptor. Binding of IL-2 is inhibited by PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤0.5 µg/test recommended
• Fluorochrome: Brilliant Ultra Violet™ 563 (BUV563), emission at 564 nm; for UV (355 nm) laser-equipped cytometers
• Light Sensitivity: Protect from light to prevent photo-induced oxidation
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in dark
• Recommended Buffer: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize polymer interactions

Excitation: 350 nm
Emission: 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The IL2 receptor complex includes IL2RA, IL2RB, and IL2RG, forming a high-affinity receptor.
IL2 regulates growth, differentiation, and apoptosis of immune cells, stimulating B cell proliferation and NK cell activity. Mutations in IL2RA are linked to immune dysfunction such as ulcerative colitis-like disease in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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