Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 737
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Series (Blue, Red, UV, Violet, Yellow variants)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright Series (436, 600, 645, 702, 780)
• Request custom conjugation

Product Specific Information

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells, particularly in bone marrow myeloid cells and memory T cells. Peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through binding to hyaluronate, an extracellular matrix component.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Recommended Buffer: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when combining polymer dye-conjugated antibodies
• Light Sensitivity: Protect from light (photo-induced oxidation sensitive)
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark
• Excitation: 350 nm
• Emission: 564 nm
• Laser: Ultraviolet Laser

Note: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD44 is a 100 kDa type I transmembrane glycoprotein expressed on human leukocytes, brain white matter, and some epithelial cells.
It acts as a receptor for hyaluronic acid (HA), mediating cell-cell interactions, adhesion, and migration.
CD44 participates in lymphocyte activation, recirculation, and homing. Expression may be upregulated in certain carcinomas and linked to metastatic potential.
Alternative splicing generates multiple isoforms with distinct functions.
Diseases associated with CD44 include superficial keratitis and lichen sclerosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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