Thermo Fisher Scientific DDX3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, HeLa, HepG2
• Predicted reactivity: Mouse (98%), Pig (97%), Chicken (84%), Bovine (97%)
• IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a member of the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and ATP-dependent RNA helicase activity. It exhibits RNA-independent ATPase activity and is stimulated by both RNA and DNA.
This protein functions in both nucleus and cytoplasm, contributing to transcriptional regulation, mRNP assembly, pre-mRNA splicing, mRNA export, translation, cellular signaling, and viral replication. Misregulation is associated with tumorigenesis.
The gene has a paralog on the Y chromosome (DDX3Y), and pseudogenes are found on chromosome 4 and X. Alternative splicing produces multiple transcript variants.

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.