Thermo Fisher Scientific HLA-AB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Flow Cytometry (Flow)

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human HLA Class 1 ABC aa 125-325 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-AB,uniProtId:P01889-1,ncbiNodeId:9606,antigenRange:125-325,antigenLength:362,antigenImageFileName:PA5-86049_HLA-AB_P01889-1_Rabbit.svg,antigenImageFileNamePDP:PA5-86049_HLA-AB_P01889-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:HLA-AB,uniProtId:P04439-1,ncbiNodeId:9606,antigenRange:125-325,antigenLength:365,antigenImageFileName:PA5-86049_HLA-AB_P04439-1_Rabbit.svg,antigenImageFileNamePDP:PA5-86049_HLA-AB_P04439-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% Glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802850

Target Information

HLA-AB refers to the human leukocyte antigen (HLA) class I molecule, specifically the HLA-A and HLA-B loci. HLA-AB plays a critical role in the immune system by presenting peptides derived from intracellular proteins to CD8+ T cells. Its widespread presence on nucleated cells allows for immune surveillance and targeted elimination of infected or abnormal cells. HLA-AB consists of a heavy chain (encoded by the HLA-A or HLA-B gene) and a beta-2 microglobulin chain. The heavy chain contains three extracellular domains: alpha-1, alpha-2, and alpha-3. These domains form a peptide-binding groove that accommodates antigenic peptides. The beta-2 microglobulin chain is non-covalently associated with the heavy chain and stabilizes the structure of the HLA-AB complex. The structure of HLA-AB enables it to bind and present a diverse range of antigenic peptides, facilitating the recognition and response to intracellular pathogens.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.