Thermo Fisher Scientific HLA-AB Polyclonal Antibody
Thermo Fisher Scientific의 HLA-AB Polyclonal Antibody는 인간 및 마우스 시료에 반응하며, Western Blot, IHC, Flow Cytometry 등 다양한 응용에 적합합니다. 고순도 Protein A 정제 방식으로 제조되어 안정적인 결과를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human HLA Class 1 ABC aa 125–325 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% Glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802850 |
Target Information
HLA-AB refers to the human leukocyte antigen (HLA) class I molecule, specifically the HLA-A and HLA-B loci.
HLA-AB plays a critical role in the immune system by presenting peptides derived from intracellular proteins to CD8+ T cells.
It is widely expressed on nucleated cells, enabling immune surveillance and targeted elimination of infected or abnormal cells.
The HLA-AB complex consists of:
- A heavy chain (encoded by HLA-A or HLA-B gene)
- A beta-2 microglobulin chain
The heavy chain contains three extracellular domains (alpha-1, alpha-2, alpha-3) forming a peptide-binding groove that accommodates antigenic peptides.
The beta-2 microglobulin chain stabilizes the structure of the HLA-AB complex.
This structure allows HLA-AB to present a diverse range of antigenic peptides, facilitating recognition and response to intracellular pathogens.
HLA and MHC antibodies are essential tools in Immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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