Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Alternative Formats Available

• APC
• APC-eFluor 780
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780

Product Specific Information

The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.

SK7 and UCHT1 antibodies cross-block binding, suggesting overlapping epitopes, whereas OKT3 and SK7 recognize distinct epitopes. The SK7 antibody recognizes chimpanzee CD3.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is suitable for cytometers equipped with a 355 nm UV laser. Confirm instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex mediates antigen recognition signaling in T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) have cytoplasmic tails that associate with signaling molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. Mutations in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for diagnostic use or resale without authorization.

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