Thermo Fisher Scientific GABRA1 Monoclonal Antibody (N95/35), PE
GABRA1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, PE 형광으로 표지되어 세포 분석에 적합함. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 4°C 보관. WB, IHC, ICC/IF 등 다양한 응용 가능.
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Thermo Fisher Scientific GABRA1 Monoclonal Antibody (N95/35), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N95/35 |
| Immunogen | Fusion protein amino acids 355–394 of human GABA-A-R-Alpha1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931942 |
Available Formats
- Unconjugated (MA5-27703)
- APC (MA5-45485)
- FITC (MA5-45486)
- PerCP (MA5-45487)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45488 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 55 kDa. No cross-reactivity against GABA-A-R-Alpha2 or -Alpha3.
Target Information
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. It causes membrane hyperpolarization through the opening of Cl⁻ channels associated with the GABAA receptor (GABAA-R) subtype.
GABAA-Rs are key therapeutic targets for sedative, anxiolytic, and hypnotic agents, and are implicated in diseases such as epilepsy, anxiety, depression, and substance abuse.
The GABAA-R is a multimeric subunit complex composed of multiple alpha, beta, and gamma subunits. Coexpression of a gamma subunit is required for benzodiazepine modulation. Different alpha subunits mediate distinct benzodiazepine effects, and phosphorylation of beta subunits can modulate receptor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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