Thermo Fisher Scientific SLC5A5 Monoclonal Antibody (FP5), APC
SLC5A5 단백질(hNIS)을 검출하는 Thermo Fisher Scientific의 단클론 항체 FP5(APC 결합형). 인간, 생쥐, 랫트 반응성. Western blot, IHC, ICC 등 다양한 응용 가능. APC 형광(651/660 nm) 특성으로 세포 분석에 적합.
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Thermo Fisher Scientific SLC5A5 Monoclonal Antibody (FP5), APC
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FP5 |
| Immunogen | Mannose binding protein hNIS fusion (AA468–643) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931946 |
Additional Formats
- FITC (Cat. No. MA5-45493)
- PerCP (Cat. No. MA5-45494)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45492 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 97 kDa, non-glycosylated version at 68 kDa. Other minor bands associated with hNIS at 160 kDa, and degradation products at approximately 30 kDa and 15 kDa.
Target Information
Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane-bound glycoprotein with 13 membrane-spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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