Thermo Fisher Scientific SLC5A5 Monoclonal Antibody (14F), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

14F

Immunogen

Mannose binding protein hNIS fusion (AA468-643) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SLC5A5,uniProtId:Q92911-1,ncbiNodeId:9606,antigenRange:468-643,antigenLength:643,antigenImageFileName:MA5-45489_SLC5A5_Q92911-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45489_SLC5A5_Q92911-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931943

Product Specific Information

1 µg/mL of MA5-45489 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approximately 30 kDa, and approximately 15kDa.

Target Information

Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.