Thermo Fisher Scientific SLC5A5 Monoclonal Antibody (14F), APC
SLC5A5 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, APC 형광 표지로 제공됩니다. Western blot, IHC, Antibody Microarray에 사용 가능하며, 사람, 생쥐, 랫트 시료에 반응합니다. 단백질 G로 정제된 액상 형태로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14F |
| Immunogen | Mannose binding protein hNIS fusion (AA468–643) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931943 |
Additional Formats
- FITC (Cat. MA5-45490)
- PerCP (Cat. MA5-45491)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45489 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 97 kDa (non-glycosylated version at 68 kDa). Other minor bands associated with hNIS observed at 160 kDa, and degradation products at approximately 30 kDa and 15 kDa.
Target Information
Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane-bound glycoprotein with 13 membrane-spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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