Thermo Fisher Scientific Phospho-Myc (Thr58) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive control: EGF-stimulated A431
• Cellular location: Nucleus

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays critical roles in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is functionally linked to cell proliferation and regulation.

c-Myc is essential for vasculogenesis and angiogenesis in tumor development. The c-Myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression can induce apoptosis.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc tags fused to target proteins.

Amplification of the c-Myc gene is observed in several human tumors (lung, breast, colon), while N-Myc amplification is common in neuroblastomas.
Chromosomal translocations involving the c-Myc locus (chromosome 8) and immunoglobulin loci (chromosomes 14, 2, or 22) are characteristic of Burkitt’s lymphoma and other B-cell proliferative disorders.
Aberrant c-Myc expression occurs in tumors of diverse origins including colorectal, gastric, hepatic, mammary, ovarian, thyroidal, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.