Thermo Fisher Scientific Phospho-Myc (Thr58) Polyclonal Antibody
인산화된 Myc (Thr58) 인식용 폴리클로날 항체로, Western blot, IHC, IP에 적합합니다. 사람 및 생쥐 시료에 반응하며, 핵 내 Myc 단백질 검출에 유용합니다. 항원 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide of human Myc with a phosphorylation site at Threonine 58 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: EGF-stimulated A431
- Cellular location: Nucleus
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays critical roles in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is functionally linked to cell proliferation and regulation.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. The c-Myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression can induce apoptosis.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc tags fused to target proteins.
Amplification of the c-Myc gene is observed in several human tumors (lung, breast, colon), while N-Myc amplification is common in neuroblastomas.
Chromosomal translocations involving the c-Myc locus (chromosome 8) and immunoglobulin loci (chromosomes 14, 2, or 22) are characteristic of Burkitt’s lymphoma and other B-cell proliferative disorders.
Aberrant c-Myc expression occurs in tumors of diverse origins including colorectal, gastric, hepatic, mammary, ovarian, thyroidal, and hematopoietic neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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