Thermo Fisher Scientific Phospho-JunD (Ser255) Polyclonal Antibody
Phospho-JunD (Ser255) 폴리클로날 항체로 인간 및 생쥐 시료에 반응. WB, IHC, ICC, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고품질 액상 항체. 세포 스트레스 반응 및 JunD 인산화 연구에 적합.
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Thermo Fisher Scientific Phospho-JunD (Ser255) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide of human JunD containing phosphorylation site Serine 255 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C for short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human mammary gland tissue and lung squamous cell carcinoma tissue
- Cellular location: Nucleus
Target Information
JunD is a transcription factor encoded by the JunD gene. Its transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N-terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) family. Jun proteins form dimers with Fos, ATF, and CREB family transcription factors to create the AP-1 complex, which regulates genes involved in growth, proliferation, differentiation, and apoptosis. JunD also plays an important role in metabolism through modulation of the IGF-1 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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