Thermo Fisher Scientific N-cadherin Monoclonal Antibody (13A9)
Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능한 N-cadherin 단일클론 항체. 인간 및 생쥐 반응성, 단백질 A 정제, 액상 형태로 제공. 세포 간 접착 및 발달 연구에 적합. -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:1,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Immunoprecipitation (IP) | 2 µg | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13A9 |
| Immunogen | MBP fusion protein containing the full cytoplasmic domain of human N-cadherin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536867 |
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion. It plays a crucial role in morphogenetic movements during development and regulates cell surface adhesion through homotypic interactions. The function of N-cadherin depends on its association with the actin cytoskeleton via interactions with cytoplasmic catenin proteins. This association is regulated by phosphorylation and dephosphorylation of beta-catenin. N-cadherin contains five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail, functioning in gastrulation and left-right asymmetry establishment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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