Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It has tyrosine phosphatase activity and is critical for lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (defined for 100 µL sample volume).
• Optimization: Antibody titration recommended for best results.
• Fluorochrome: Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm, for use with UV (355 nm) laser-equipped cytometers.
• Buffer Recommendations: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
• Excitation/Emission: 350 nm / 395 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and plays a vital role in T- and B-cell receptor signaling.
CD45 isoforms differ due to alternative splicing and are involved in cell signaling, survival, and immune regulation. It is absent in non-hematopoietic tissues and cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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