Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 395, eBioscience
마우스 CD45를 인식하는 30-F11 단클론 항체로, Brilliant Ultra Violet 395 형광체로 표지되어 있습니다. 유세포분석용으로 검증되었으며, 모든 조혈세포에서 발현되는 CD45 검출에 적합합니다. 4°C 보관, 동결 금지. 연구용 전용 시약입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1.0 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet™ 395, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Brilliant Ultra Violet™ 395 |
| Excitation/Emission Max | 347 / 399 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925263 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It has tyrosine phosphatase activity and is critical for lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (defined for 100 µL sample volume).
- Optimization: Antibody titration recommended for best results.
- Fluorochrome: Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm, for use with UV (355 nm) laser-equipped cytometers.
- Buffer Recommendations: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
- Excitation/Emission: 350 nm / 395 nm
- Laser: Ultraviolet
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and plays a vital role in T- and B-cell receptor signaling.
CD45 isoforms differ due to alternative splicing and are involved in cell signaling, survival, and immune regulation. It is absent in non-hematopoietic tissues and cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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