Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, Alexa Fluor 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV 496/563/615/737
• Brilliant Violet 421/605/650/711
• eFluor 450/506, FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow, Violet, UV series
• PE, PE-Cyanine5/7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436/600/645/702/780
• Request custom conjugation

Product Specific Information

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule by binding to hyaluronate, an extracellular matrix component.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes (≤1.0 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test)
• Fluorochrome Information: Brilliant Ultra Violet™ 395 emits at 395 nm and is designed for cytometers with an ultraviolet (355 nm) laser
• Recommended Buffers: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD44 is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leukocytes, brain white matter, and certain epithelial cells. It acts as a receptor for hyaluronic acid (HA) and mediates cell-cell interactions, adhesion, and migration. CD44 is involved in lymphocyte activation, recirculation, and homing, and may be upregulated in carcinomas associated with metastatic potential. Multiple isoforms exist due to alternative splicing, contributing to functional diversity. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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