Thermo Fisher Scientific Streptavidin Polyclonal Antibody, HRP

Applications

• Western Blot (WB): 1:1,000
• ELISA: 1:2,000–1:10,000

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin (Streptomyces avidinii). No cross-reactivity occurs against avidin.

Target Information

Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin (Kd ≈ 10⁻¹⁴ mol/L).
The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.