Thermo Fisher Scientific vPARP Polyclonal Antibody
Rabbit polyclonal antibody recognizing human vPARP, suitable for WB, IHC, and IP applications. Recommended dilutions: WB 1:2,000–1:10,000, IHC 1:200–1:1,000. Supplied as liquid, 0.2 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1674 to 1724 of Human vault poly(ADP-ribose) polymerase (vPARP, UniProt ID: Q9UKK3-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, capable of catalyzing a poly(ADP-ribosyl)ation reaction. The protein includes a catalytic domain homologous to poly(ADP-ribosyl) transferase but lacks the N-terminal DNA binding domain that activates the C-terminal catalytic domain. Since it cannot bind DNA directly, its transferase activity may be activated by other factors such as protein–protein interactions mediated by the extensive carboxyl terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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