
Thermo Fisher Scientific EWSR1 Monoclonal Antibody (OTI2D9)
EWSR1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 적용 가능. 인간 시료 반응성. 동결건조 형태로 제공되며, PBS + 8% trehalose buffer에 보존. 연구용으로 Ewing sarcoma 관련 단백질 분석에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 256–447 of human EWSR1 (NP_005234) produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The EWSR1 gene encodes a multifunctional protein involved in gene expression, cell signaling, RNA processing, and transport.
It contains an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain.
Chromosomal translocations between EWSR1 and transcription factor genes can produce chimeric proteins associated with tumorigenesis, including Ewing sarcoma and other neuroectodermal tumors.
Alternative splicing results in multiple transcript variants, and pseudogenes are found on chromosomes 1 and 14.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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