Thermo Fisher Scientific EWSR1 Monoclonal Antibody (OTI3F6)
EWSR1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, 인간 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 고순도 친화 크로마토그래피 정제 제품입니다. 라이오필라이즈 형태로 제공되어 안정적이며 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 256–447 of human EWSR1 (NP_005234) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The EWSR1 gene encodes a multifunctional protein involved in gene expression, cell signaling, RNA processing, and transport. It contains an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain.
Chromosomal translocations between EWSR1 and other transcription factor genes lead to chimeric proteins implicated in tumorigenesis, including Ewing sarcoma, neuroectodermal tumors, and other malignancies.
Alternative splicing produces multiple transcript variants, and related pseudogenes are found on chromosomes 1 and 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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