Thermo Fisher Scientific GCLM Polyclonal Antibody
GCLM 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. Human에 반응하며 Mouse, Rat, Bovine에서도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 항체로, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 29–120 of Human GCLM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546860 |
Product Specific Information
- Recommended positive controls: HeLa, 293T, A431, H1299, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate-limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH acts as a vital intra- and extracellular antioxidant. Gamma-GCS is a heterodimer consisting of a heavy catalytic subunit and a light regulatory subunit, responsive to inflammation, phenolic antioxidants, heat shock, oxidants, and cytokines.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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