Thermo Fisher Scientific Mouse IFN-alpha A Recombinant Protein
Thermo Fisher Scientific의 Mouse IFN-alpha A 재조합 단백질은 항바이러스 활성 평가용으로 사용됩니다. E. coli에서 발현된 고순도(≥95%) 단백질로, 다양한 면역학적 및 기능적 분석(ELISA, WB, IHC 등)에 적합합니다. -80°C에서 보관하며 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Mouse |
| Expression System | E. coli |
| Molecular Weight | 19.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% |
| Activity | Specific Activity = 7.25×10⁷ units/mg; Biological activity determined by cytopathic effect inhibition assay (Rubinstein et al., 1981). 1 unit/mL of interferon inhibits cytopathic effect by 50% using L929 cells challenged with encephalomyocarditis virus. Units are standardized to NIH reference (Pestka, 1986). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | No preservative |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
Product Specific Information
- Dilution Recommendation: Use polypropylene tubes and pipet tips when diluting this interferon.
- Further dilutions should be made in a buffered solution containing a carrier protein such as BSA.
- Optimal concentration should be determined for each specific application.
Target Information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. Interaction of IFN-alpha with its receptor activates multiple signaling pathways involved in innate immune response against viral infection.
In humans, the IFN alpha gene is located on chromosome 9. Type I interferons include 14 alpha isoforms and single beta, omega, epsilon, and kappa isoforms. Homologous molecules are found in various species including mice, rats, birds, reptiles, and fish.
IFN alpha is primarily produced by plasmacytoid dendritic cells and plays a key role in antiviral innate immunity and modulation of adaptive immunity. Dysfunctions of IFN alpha are associated with diseases such as Hepatitis C and Hepatitis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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