Thermo Fisher Scientific Mouse IFN-alpha A Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

E. coli

Molecular weight

19.3 kDa

Class

Recombinant

Type

Protein

Purity

≥95%

Activity

Specific Activity = 7.25x10e7 units/mg; The biological activity is determined by titration with the use of the cytopathic effect inhibition assay (Rubinstein et al., 1981). In this antiviral assay for interferon, about 1 unit/mL of interferon is the quantity necessary to inhibit the cytopathic effect by 50% using L929 cells challenged with encephalomyocarditis virus. The units are determined with respect to the international reference standard for mouse interferon alpha (mouse-IFN-α) provided by the National Institutes of Health (Pestka, 1986).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Dilution Recommendation: Polypropylene tubes and pipet tips should be used when diluting this interferon. Further dilutions should be made in buffered solution containing a carrier protein, such as BSA. The optimal concentration should be determined for each specific application.

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.