Thermo Fisher Scientific Mouse LIF Recombinant Protein

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

E. coli

Amino acid sequence

Mouse LIF recombinant protein contains 181 amino acids

Molecular weight

20 kDa

Class

Recombinant

Type

Protein

Purity

≥95% by SDS-PAGE

Endotoxin concentration

≤1 EU/µg

Activity

ED50 ≤ 1 ng/mL; determined by the dose-dependent production of IL-6 in M1 myeloid leukemic cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS with 0.02% Tween 20

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Storage: Store at -20°C to -80°C for 12 months, 1 month at 4°C after reconstituted or 3 months when stored at -20°C to -80°C after reconstituting as directed.

Target Information

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.