Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9B1)
Vimentin 단백질을 인식하는 Mouse monoclonal 항체(V9B1)로, Western blot 및 IHC(P) 분석에 적합합니다. Human 시료에서 반응하며, Protein A로 정제된 0.5 mg/mL 액상 제품입니다. 세포 내 핵 및 세포질 위치 확인용으로 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V9B1 |
| Immunogen | Recombinant protein of human Vimentin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Melanoma, Carcinoma
- Cellular location: Nuclear, cytoplasmic
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFPs, vimentin is expressed along with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin also plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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