Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (ZY428)
DYKDDDDK(FALG) 태그 단백질 검출용 마우스 단클론 항체. Western blot, ICC/IF, IP 등 다양한 응용에 적합. 고순도 Protein A 정제, 0.5 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZY428 |
| Immunogen | A synthetic peptide DYKDDDDK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Positive control: HEK-293T cells transfected with DDDDK-tagged human PD-L1.
Target Information
The DYKDDDDK tag, also known as the FLAG tag, is a short peptide sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used as a protein tag to facilitate purification, detection, and localization. It can be fused to the N- or C-terminus of a protein, allowing easy identification and isolation using anti-FLAG antibodies.
This tag is widely used in molecular biology and biochemistry for protein-protein interaction studies, expression analysis, and localization studies. Its hydrophilic nature minimizes interference with protein folding or activity.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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