Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (ZY428)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

2-5 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ZY428

Immunogen

A synthetic peptide DYKDDDDK

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: HEK-293T transfected with DDDDK-tagged human PD-L1.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.