Thermo Fisher Scientific BRSK1 Polyclonal Antibody
Rabbit polyclonal antibody targeting BRSK1 for WB and IHC applications. Reacts with human, mouse, and rat. Lyophilized form, reconstitute in sterile water. Suitable for research use in neuronal and DNA damage checkpoint studies.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from aa region 710–750 of mouse BRSK1 conjugated to an immunogenic carrier protein was used as the antigen. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: BRSK1.
Target Information
BRSK1 (SAD1) is a serine/threonine kinase involved in UV-induced DNA damage checkpoint regulation. It is highly expressed in the brain and testes and plays a role in stress-induced cell cycle arrest. Overexpression leads to G2/M arrest in HeLa S2 cells, while reduced expression can partially abrogate UV-induced G2/M arrest, highlighting its role in DNA damage checkpoint function.
BRSK1 and its homolog BRSK2 are essential for mammalian neuronal polarization. Double-mutant mice lacking both BRSK1 and BRSK2 die shortly after birth, and their neurons exhibit uniformly sized neurites with mixed axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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