Thermo Fisher Scientific pro BDNF Polyclonal Antibody
Rabbit polyclonal antibody targeting human proBDNF for WB and IHC applications. Recognizes proBDNF specifically. Supplied lyophilized, unconjugated, and preservative-free. Suitable for research use in neuronal survival and differentiation studies.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | 1:1,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human proBDNF conjugated to blue carrier protein was used as the antigen |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: proBDNF.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form.
BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons.
Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated.
BDNF promotes neuronal survival and differentiation and plays a critical role in memory formation and synaptic regulation.
Expression of BDNF is reduced in both Alzheimer’s and Huntington disease patients, and may also play a role in the regulation of stress response and mood disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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