Thermo Fisher Scientific HDAC1 Monoclonal Antibody (OTI5C3), TrueMAB
HDAC1 단백질을 표적으로 하는 Mouse monoclonal antibody (Clone OTI5C3). Western blot, ICC/IF, Flow cytometry에 적합. Human 및 Rat 반응성. Lyophilized 형태로 보관 용이하며, 재구성 후 1 mg/mL 농도로 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C3 |
| Immunogen | Full length human recombinant HDAC1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, are crucial for regulating eukaryotic gene expression.
HDAC1 belongs to the histone deacetylase family and is part of the histone deacetylase complex.
It interacts with the retinoblastoma tumor-suppressor protein, playing a key role in controlling cell proliferation and differentiation.
Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effects on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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