Thermo Fisher Scientific Histone H3 Recombinant Rabbit Monoclonal Antibody (17H2L9), Alexa Fluor 647
Alexa Fluor 647로 표지된 재조합 토끼 단클론 항히스톤 H3 항체로, Western blot과 면역세포염색(ICC/IF)에 적합합니다. 높은 특이성과 재현성을 제공하며, PBS/BSA 완충액에 보관됩니다. 4°C에서 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 3–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 17H2L9 |
| Immunogen | Synthetic peptide corresponding to the N-terminal region of human Histone H3 (aa 4–11) |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2663072 |
Additional Formats
Product Specific Information
Since Histone H3 is highly conserved across species, this antibody may react with multiple species.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA. Histone H3 plays a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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