Thermo Fisher Scientific Histone H3 Recombinant Rabbit Monoclonal Antibody (17H2L9), Alexa Fluor 555
Histone H3 단백질 검출용 Alexa Fluor 555 결합 재조합 토끼 단클론 항체. Western blot 및 면역세포염색에 최적화. 높은 특이성과 감도를 제공하며, 로트 간 일관성 유지. 인간, 생쥐, 랫트 반응성.
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Thermo Fisher Scientific Histone H3 Recombinant Rabbit Monoclonal Antibody (17H2L9), Alexa Fluor 555
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 17H2L9 |
| Immunogen | Synthetic peptide corresponding to the N-terminal region of human Histone H3 (aa 4–11) |
| Conjugate | Alexa Fluor™ 555 |
| Excitation / Emission Max | 553 / 568 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2663070 |
Additional Formats
Product Specific Information
Since Histone H3 is highly conserved across species, this antibody may react with multiple species.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in eukaryotic chromatin. Together with other core histones, it forms nucleosomes that organize DNA structure.
Histone H3 plays key roles in transcription regulation, DNA repair, replication, and chromosomal stability.
Its N-terminal tail undergoes diverse post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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