Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-30S
• NovaFluor Blue 660-40S
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The A20 monoclonal antibody reacts with mouse CD45 (leukocyte common antigen, LCA) in CD45.1-expressing mouse strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration recommended.
• Fluorochrome: Brilliant Ultra Violet™ 805 (BUV805), emits at 797 nm, for cytometers with 355 nm UV laser.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific polymer interactions.
• Light Sensitivity: Protect from light.
• Fixation: Compatible with IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333). Store up to 3 days at 4°C in dark.
• Methanol Compatibility: Short-term methanol fixation OK; avoid >1 hour exposure.
• Excitation/Emission: 355 nm / 797 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, critical for lymphocyte development and antigen signaling. CD45 exists in multiple isoforms via alternative splicing, differing in extracellular domains. It also modulates integrin-mediated signaling and is involved in apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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