Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), Super Bright 600, eBioscience

Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184, Fusin, LESTR, HUMSTR). CXCR4 is a G-protein-coupled chemokine receptor functioning as a co-receptor for X4 HIV-1 entry into CD4+ cells and plays a key role in lymphocyte migration. Its ligand is SDF1, and it is essential for B cell development and maintenance of granulocyte progenitors in bone marrow.

The 2B11 antibody recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1a binding.
Tested for flow cytometric analysis of mouse thymocytes, recommended ≤1.0 µg/test (100 µL final volume). Optimal titration is advised.

Super Bright 600 is a tandem dye excited at 405 nm and emits at 600 nm; use a 610/20 bandpass filter.
For multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.

• Light sensitivity: Protect from light.
• Fixation: Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
• Excitation: 405 nm
• Emission: 600 nm
• Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a seven-transmembrane G-protein-coupled receptor acting as a co-receptor for HIV-1 and HIV-2 entry into CD4+ cells. It binds the chemokine CXCL12/SDF-1, mediating calcium signaling and MAPK activation. CXCR4 also interacts with extracellular ubiquitin, influencing cAMP levels and calcium flux.

It plays essential roles in hematopoiesis, cardiac septum formation, and gastrointestinal vascularization. In the CNS, CXCR4 mediates hippocampal neuron survival and cerebellar development. It binds bacterial LPS and mediates inflammatory TNF responses. Antibodies against CXCR4 block HIV-1 and HIV-2 infection of human target cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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