Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), Super Bright 600, eBioscience
CXCR4/CD184을 인식하는 2B11 클론 단일클론 항체로, Super Bright 600 형광체가 결합되어 있습니다. 마우스 및 인간 CXCR4 검출에 적합하며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 높은 특이성과 안정적인 형광 신호를 제공합니다.
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Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B11 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688142 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 805
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184, Fusin, LESTR, HUMSTR). CXCR4 is a G-protein-coupled chemokine receptor functioning as a co-receptor for X4 HIV-1 entry into CD4+ cells and plays a key role in lymphocyte migration. Its ligand is SDF1, and it is essential for B cell development and maintenance of granulocyte progenitors in bone marrow.
The 2B11 antibody recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1a binding.
Tested for flow cytometric analysis of mouse thymocytes, recommended ≤1.0 µg/test (100 µL final volume). Optimal titration is advised.
Super Bright 600 is a tandem dye excited at 405 nm and emits at 600 nm; use a 610/20 bandpass filter.
For multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
- Light sensitivity: Protect from light.
- Fixation: Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
- Excitation: 405 nm
- Emission: 600 nm
- Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CXCR4 (Fusin, LESTR, HUMSTR) is a seven-transmembrane G-protein-coupled receptor acting as a co-receptor for HIV-1 and HIV-2 entry into CD4+ cells. It binds the chemokine CXCL12/SDF-1, mediating calcium signaling and MAPK activation. CXCR4 also interacts with extracellular ubiquitin, influencing cAMP levels and calcium flux.
It plays essential roles in hematopoiesis, cardiac septum formation, and gastrointestinal vascularization. In the CNS, CXCR4 mediates hippocampal neuron survival and cerebellar development. It binds bacterial LPS and mediates inflammatory TNF responses. Antibodies against CXCR4 block HIV-1 and HIV-2 infection of human target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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