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Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Super Bright 600, eBioscience
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Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Super Bright 600, eBioscience

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인간 IgM의 μ 중쇄를 특이적으로 인식하는 SA-DA4 단클론 항체. Super Bright 600 형광체로 결합되어 유세포분석(Flow Cytometry)에 최적화. 높은 형광 효율과 낮은 비특이적 결합. 연구용으로만 사용 가능.

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Thermo Fisher Scientific 63-9998-42 IgM Monoclonal Antibody (SA-DA4), Super Bright 600, eBioscience 100 tests pk판매 단위 pk ·
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Thermo Fisher Scientific 63-9998-41 IgM Monoclonal Antibody (SA-DA4), Super Bright 600, eBioscience 25 tests pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Super Bright 600, eBioscience

Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Super Bright 600, eBioscience

Applications and Tested Dilution

  • Application: Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

항목 내용
Host / Isotype Mouse / IgG1, kappa
Recommended Isotype Control Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™
Class Monoclonal
Type Antibody
Clone SA-DA4
Conjugate Super Bright™ 600
Excitation / Emission Max 414 / 601 nm
Form Liquid
Concentration 5 µL/Test
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Wet ice
RRID AB_2662358

Additional Formats

  • Unconjugated
  • APC
  • Biotin
  • Brilliant UV 395
  • Brilliant UV 737
  • Brilliant Violet 480
  • Brilliant Violet 605
  • Brilliant Violet 786
  • eFluor 450
  • FITC
  • PE
  • PE-Cyanine7
  • PerCP-eFluor 710
  • Super Bright 436
  • Request custom conjugation

Product Specific Information

The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM and does not cross-react with other immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly during early B cell development and on the surface of mature B cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.125 µg) per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples may be stored up to 3 days at 2–8°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.

Excitation / Emission / Laser:
405 nm / 600 nm / Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells in the bone marrow and peripheral blood.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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