Thermo Fisher Scientific Beclin 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Immunohistochemistry (Frozen) (IHC (F))

1:400

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

2-10 µg/mL

-

Immunoprecipitation (IP)

1:80

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide to human Beclin 1, containing residues within 1-100. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Beclin 1,uniProtId:Q14457-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:450,antigenImageFileName:PA1-16857_Beclin_1_Q14457-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16857_Beclin_1_Q14457-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568459

Product Specific Information

The target sequence has 88% sequence homology with rat.

Suggested positive control: antigen standard for BECN1 (transient overexpression lysate).

Target Information

Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.