Thermo Fisher Scientific Beclin 1 Polyclonal Antibody
Beclin 1 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인간·마우스·랫트 반응성. Western blot, IHC, ICC, Flow cytometry 등 다중 응용 가능. 항원 친화 크로마토그래피로 정제된 1 mg/mL 액상 시약으로, 자가포식 연구에 적합.
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Thermo Fisher Scientific Beclin 1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:400 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 1:80 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide to human Beclin 1, residues 1–100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568459 |
Product Specific Information
- The target sequence has 88% sequence homology with rat.
- Suggested positive control: antigen standard for BECN1 (transient overexpression lysate).
Target Information
Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.
Different PI3K complex forms are believed to participate in multiple membrane trafficking pathways:
- PI3KC3-C1 is involved in the initiation of autophagosomes.
- PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis.
Beclin 1 also plays a role in antiviral host defense.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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