Thermo Fisher Scientific ATM Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval is not recommended.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. The ATM gene is associated with ataxia-telangiectasia, a rare autosomal recessive disorder characterized by progressive neurological degeneration, immunologic deficiency, and increased risk of lymphoid cancer.

ATM phosphorylates proteins rather than lipids and has multiple downstream targets such as P53, Mdm2, BRCA1, and SMC1, which act as cell-cycle regulators. The ATM protein plays a critical role in repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens.

ATM’s C-terminal region shares homology with catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). It becomes autophosphorylated and upregulated upon exposure to ionizing radiation. ATM-deficient cells are hypersensitive to radiation, show impaired DNA synthesis inhibition, and delayed p53 induction. DNA damage triggers ATM-kinase activation, leading to a cascade of kinase reactions regulating cell cycle, apoptosis, and DNA damage repair.

Studies have also linked ATM to apoptosis regulation along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.