Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI1D4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:4,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50–1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a Ca²⁺-independent adhesion molecule and influences cell cycle and proliferation through induction of c-myc, cyclin A, and cyclin E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a marker for carcinomas of epithelial origin.
Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.