Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI2D9), TrueMAB
인간 EpCAM 단백질을 표적으로 하는 Mouse IgG1 단클론 항체. Western blot 및 면역세포화학 실험에 적합. 동결건조 형태로 제공되며 PBS, trehalose 완충액에 저장. EpCAM 관련 세포 접착 및 종양 연구용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:4,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D9 |
| Immunogen | Full length human recombinant protein of human EPCAM produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues.
It functions as a calcium-independent cell adhesion molecule, influencing cell cycle and proliferation through induction of c-myc, cyclin A, and E.
Ep-CAM-mediated adhesion negatively regulates cadherin-based adhesion, affecting epithelial differentiation and growth.
Overexpression is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial lineage.
Ep-CAM is expressed on baso-lateral surfaces of simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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