Thermo Fisher Scientific mPR alpha Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel membrane progestin receptor (mPR). This pathway inhibits adenylyl cyclase, reduces intracellular cAMP, and activates mitogen-activated protein kinase to effect signal transduction pathways.
Three distinct groups, designated α, β, and γ, comprise this gene family.
mPRα, also designated progestin and adipoQ receptor family member VII (PAQR7), consists of an extracellular N-terminus, an intracellular C-terminus, and seven transmembrane domains. It is expressed in ovary, testis, placenta, uterus, and bladder.
mPRβ, also designated progestin and adipoQ receptor family member VIII (PAQR8), consists of eight putative transmembrane regions and an intracellular N-terminus that contains a leucine-rich motif. It is a 354 amino acid protein with a molecular mass of about 40 kDa and is expressed in brain and spinal cord.
Both mPRα and mPRβ may be G protein-coupled receptors and may be involved in oocyte maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.