Thermo Fisher Scientific RENT1/hUPF1 Polyclonal Antibody
RENT1/hUPF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 인간, 마우스, 랫트 반응성. IHC, ICC/IF, ELISA 등 다양한 응용 가능. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human RENT1/hUPF1, amino acids 301–450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Upf1 is a key component of nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. It functions as an ATP-dependent RNA helicase in the cytoplasm and is a component of cytoplasmic P-bodies. Phosphorylation of Upf1 mediates translation repression associated with NMD, facilitating mRNA accessibility to the decay machinery. Other active components of NMD include Upf2 and Upf3, which exhibit perinuclear and nucleocytoplasmic localization, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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