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Norgen Urine DNA Isolation Kit

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Rapid and reliable isolation of genomic and apoptotic DNA from urine samples; compatible with various volumes and formats; removes PCR inhibitors for high-quality DNA suitable for PCR, qPCR, and viral DNA detection; ideal for molecular diagnostics and ...

카탈로그번호: 18100
브랜드: Norgen
카테고리: Genomic DNA Purification Systems and Kits
판매단위: pk

#Norgen #R #DNA Reagents #Reagents

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Norgen · Norgen Urine DNA Isolation Kit

Norgen Urine DNA Isolation Kit

SKU: 18100

Fast and reliable purification of genomic and apoptotic DNA from urine.

Features and Benefits

Rapid isolation of both small and large DNA species from urine
Convenient spin column format
Effective removal of PCR inhibitors
Purified DNA suitable for sensitive downstream applications (PCR, qPCR, DNA fingerprinting, methylation studies)
Enables purification of viral DNA from urine

Both high molecular weight DNA (>1 kb, cell-associated) and small DNA (150–250 bp, circulating) are efficiently isolated using a rapid spin column protocol. Salts, proteins, and other contaminants are removed to yield inhibitor-free DNA.

This kit is compatible with Norgen’s Urine Collection and Preservation Tubes.

Kit Variants

Urine DNA Isolation Kit

For 50 μL–1.75 mL urine samples
Preparation time: ~30 minutes per sample

Urine DNA Isolation Kit (Slurry Format)

For 3–25 mL urine samples
Preparation time: <30 minutes per sample

Urine DNA Isolation Maxi Kit (Slurry Format)

For 25–80 mL urine samples
Preparation time: <30 minutes per sample

Background

Urine DNA consists of:

Genomic DNA (gDNA): >1 kb, derived from exfoliated cells
Apoptotic DNA: 150–250 bp, partly from circulation and may include RNA/DNA hybrids
Both species can be reliably isolated using this kit.

Supporting Data

Figure 1. A Typical Agarose Gel Showing Total Urinary DNA Isolated from 1.5 mL of Urine.
DNA was isolated using the bind, wash, and elute procedure. Two elutions (E1: 100 µL, E2: 75 µL) were analyzed on a 1.2% agarose gel. Both large and small DNA species are visible.

추출된 이미지 텍스트:
| Lane | Label |
|------|------------|
| M | Marker |
| | Sample 1 |
| | E1 |
| | E2 |
| | Sample 2 |
| | E1 |
| | E2 |

DNA size markers (bp): 2000, 1500, 1000, 750, 500, 300, 150, 50

Figure 2. Isolation and Detection of DNA from 1.75 mL Urine Samples.
DNA was isolated and analyzed by qPCR using human 5S primers. The graph shows amplification curves for different elutions.

추출된 이미지 텍스트:

Y축: PCR Base Line Subtracted CF RFU
X축: Cycle number (16–48)
여러 색상의 곡선으로 PCR 증폭 곡선 표시

Figure 3. Circulating DNA Isolated from Urine Used as PCR Template.
DNA from urine was amplified for K-ras gene detection (157 bp product). Lanes A–C: samples; D: positive control; E: negative control; M: marker.

추출된 이미지 텍스트:
| Lane | Label |
|------|--------|
| M | Marker |
| A | Sample 1 |
| B | Sample 2 |
| C | Sample 3 |
| D | Positive Control |
| E | Negative Control |
| M | Marker |

DNA size markers (bp): 2000, 1500, 1000, 750, 500, 300, 150, 50

Figure 4. Total Urinary DNA Isolated from Different Urine Volumes (Slurry Format).
DNA isolated from 3 mL, 6 mL, 12 mL, and 24 mL urine samples. Most DNA appears in the first elution.

추출된 이미지 텍스트:
| Volume | Elutions |
|---------|-----------|
| 3 mL | E1, E2 |
| 6 mL | E1, E2 |
| 12 mL | E1, E2 |
| 24 mL | E1, E2 |
| M | Marker |

Figure 5. Isolation and Detection of DNA from Different Urine Volumes.
qPCR analysis confirms successful DNA detection without PCR inhibition.

추출된 이미지 텍스트:

Y축: PCR Base Line Subtracted CF RFU
X축: Cycle (16–40)
여러 색상의 증폭 곡선 표시

Figure 6. Highly Sensitive Isolation of Viral DNA from 25 mL Urine.
HSV-1 DNA detected down to 250 viral copies using PCR amplification (275 bp product).

Figure 7. Total Urinary DNA Isolated from Different Urine Volumes (Maxi Kit).
DNA yield increases linearly with urine input volume.

Figure 8. Linear Decrease of Ct Values with Increasing Urine Input.
qPCR confirms linearity between DNA yield and urine volume.

Kit Specifications

Specification	Detail
Volume of Urine Processed	1.75 mL
Average Yield*	Up to 50 ng
Size of DNA Purified	Large (>1 kb) and small (150–250 bp)
Time for 10 Purifications	30 min hands-on + 1 hr incubation

* Yield varies by sample type.

Storage Conditions and Stability

All buffers should be stored sealed at room temperature. Stable for 2 years after shipment. Proteinase K is ready-to-use and stable for 2 years at room temperature.

Kit Components

Component	Cat. 18100 (50 preps)	Cat. 48800 (50 preps)	Cat. 50100 (50 preps)
Binding Solution K	15 mL	–	–
Slurry B1	–	18 mL	18 mL
Proteinase K in Storage Buffer	2 mL	–	–
Pronase K in Storage Buffer	2 mL	–	–
Solution WN	9 mL	–	–
Binding Buffer A	–	–	50 mL
Lysis Buffer A	–	30 mL	30 mL
Wash Solution A	–	38 mL	38 mL
Wash Solution B	30 mL	–	–
Wash Solution D	9 mL	–	–
Elution Buffer B	15 mL	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Filter Spin Columns	–	50	50
Collection Tubes	50	50	50
Elution Tubes (1.7 mL)	100	100	100
Product Insert	1	1	1

Documentation

Protocols

Application Notes

Safety Data Sheets

FAQs (Selected)

Sample Storage:
Use Norgen’s Urine Preservative for nucleic acid and protein preservation at ambient temperature.

Centrifugation:
Fixed-speed centrifuge may reduce yields; perform all steps at room temperature.

Processing Variable Volumes:
Protocols can be adjusted for 50 μL–2.6 mL urine by scaling reagent volumes.

Frozen Samples:
Thaw gently at room temperature or 37°C for 5 min; do not centrifuge before processing.

Citations

Recent publications using Norgen’s Urine DNA Isolation Kits include studies in Microbiology Spectrum (2024), Equine Veterinary Journal (2023), Microorganisms (2023), Viruses (2023), mBio (2016), Scientific Reports (2015), and others.

제품 이미지

Figure 1. A Typical Agarose Gel Showing Total Urinary DNA
Figure 2. Isolation and Detection of DNA from 1.75 mL Urine Samples
Figure 3. Circulating DNA Isolated from Urine
Figure 4. Isolation of Total Urinary DNA from Different Urine Volumes
Figure 5. Isolation and Detection of DNA from Different Urine Volumes
Figure 6. Highly Sensitive Isolation of Viral DNA
Figure 7. Total Urinary DNA Isolated from Different Urine Volumes
Figure 8. Linear Decrease of Ct Values with Increasing Urine Input