Norgen Urine DNA Isolation Kit

Norgen Urine DNA Isolation Kit

SKU: 18100

Fast and reliable purification of genomic and apoptotic DNA from urine.

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Features and Benefits

• Rapid isolation of both small and large DNA species from urine
• Convenient spin column format
• Effective removal of PCR inhibitors
• Purified DNA suitable for sensitive downstream applications (PCR, qPCR, DNA fingerprinting, methylation studies)
• Enables purification of viral DNA from urine

Both high molecular weight DNA (>1 kb, cell-associated) and small DNA (150–250 bp, circulating) are efficiently isolated using a rapid spin column protocol. Salts, proteins, and other contaminants are removed to yield inhibitor-free DNA.

This kit is compatible with Norgen’s Urine Collection and Preservation Tubes.

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Kit Variants

Urine DNA Isolation Kit

• For 50 μL–1.75 mL urine samples
• Preparation time: ~30 minutes per sample

Urine DNA Isolation Kit (Slurry Format)

• For 3–25 mL urine samples
• Preparation time: <30 minutes per sample

Urine DNA Isolation Maxi Kit (Slurry Format)

• For 25–80 mL urine samples
• Preparation time: <30 minutes per sample

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Background

Urine DNA consists of:

• Genomic DNA (gDNA): >1 kb, derived from exfoliated cells
• Apoptotic DNA: 150–250 bp, partly from circulation and may include RNA/DNA hybrids
  Both species can be reliably isolated using this kit.

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Supporting Data

Figure 1. A Typical Agarose Gel Showing Total Urinary DNA Isolated from 1.5 mL of Urine.
DNA was isolated using the bind, wash, and elute procedure. Two elutions (E1: 100 µL, E2: 75 µL) were analyzed on a 1.2% agarose gel. Both large and small DNA species are visible.

추출된 이미지 텍스트:
| Lane | Label |
|------|------------|
| M | Marker |
| | Sample 1 |
| | E1 |
| | E2 |
| | Sample 2 |
| | E1 |
| | E2 |

DNA size markers (bp): 2000, 1500, 1000, 750, 500, 300, 150, 50

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Figure 2. Isolation and Detection of DNA from 1.75 mL Urine Samples.
DNA was isolated and analyzed by qPCR using human 5S primers. The graph shows amplification curves for different elutions.

추출된 이미지 텍스트:

• Y축: PCR Base Line Subtracted CF RFU
• X축: Cycle number (16–48)
• 여러 색상의 곡선으로 PCR 증폭 곡선 표시

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Figure 3. Circulating DNA Isolated from Urine Used as PCR Template.
DNA from urine was amplified for K-ras gene detection (157 bp product). Lanes A–C: samples; D: positive control; E: negative control; M: marker.

추출된 이미지 텍스트:
| Lane | Label |
|------|--------|
| M | Marker |
| A | Sample 1 |
| B | Sample 2 |
| C | Sample 3 |
| D | Positive Control |
| E | Negative Control |
| M | Marker |

DNA size markers (bp): 2000, 1500, 1000, 750, 500, 300, 150, 50

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Figure 4. Total Urinary DNA Isolated from Different Urine Volumes (Slurry Format).
DNA isolated from 3 mL, 6 mL, 12 mL, and 24 mL urine samples. Most DNA appears in the first elution.

추출된 이미지 텍스트:
| Volume | Elutions |
|---------|-----------|
| 3 mL | E1, E2 |
| 6 mL | E1, E2 |
| 12 mL | E1, E2 |
| 24 mL | E1, E2 |
| M | Marker |

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Figure 5. Isolation and Detection of DNA from Different Urine Volumes.
qPCR analysis confirms successful DNA detection without PCR inhibition.

추출된 이미지 텍스트:

• Y축: PCR Base Line Subtracted CF RFU
• X축: Cycle (16–40)
• 여러 색상의 증폭 곡선 표시

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Figure 6. Highly Sensitive Isolation of Viral DNA from 25 mL Urine.
HSV-1 DNA detected down to 250 viral copies using PCR amplification (275 bp product).

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Figure 7. Total Urinary DNA Isolated from Different Urine Volumes (Maxi Kit).
DNA yield increases linearly with urine input volume.

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Figure 8. Linear Decrease of Ct Values with Increasing Urine Input.
qPCR confirms linearity between DNA yield and urine volume.

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Kit Specifications

Specification	Detail
Volume of Urine Processed	1.75 mL
Average Yield*	Up to 50 ng
Size of DNA Purified	Large (>1 kb) and small (150–250 bp)
Time for 10 Purifications	30 min hands-on + 1 hr incubation

* Yield varies by sample type.

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Storage Conditions and Stability

All buffers should be stored sealed at room temperature. Stable for 2 years after shipment. Proteinase K is ready-to-use and stable for 2 years at room temperature.

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Kit Components

Component	Cat. 18100 (50 preps)	Cat. 48800 (50 preps)	Cat. 50100 (50 preps)
Binding Solution K	15 mL	–	–
Slurry B1	–	18 mL	18 mL
Proteinase K in Storage Buffer	2 mL	–	–
Pronase K in Storage Buffer	2 mL	–	–
Solution WN	9 mL	–	–
Binding Buffer A	–	–	50 mL
Lysis Buffer A	–	30 mL	30 mL
Wash Solution A	–	38 mL	38 mL
Wash Solution B	30 mL	–	–
Wash Solution D	9 mL	–	–
Elution Buffer B	15 mL	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Filter Spin Columns	–	50	50
Collection Tubes	50	50	50
Elution Tubes (1.7 mL)	100	100	100
Product Insert	1	1	1

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Documentation

Protocols

• (18100) Urine DNA Isolation Kit - Protocol (50 prep)
• (48800) Urine DNA Isolation Kit (Slurry Format) - Protocol (50 prep)
• (50100) Urine DNA Isolation Maxi Kit (Slurry Format) - Protocol (50 prep)

Application Notes

• Isolation of DNA From As Little As 25 μL of Urine
• Isolation of High Quality Urinary DNA
• Determination of DNA Molecular Weight

Safety Data Sheets

• 18100 - Urine DNA Isolation Kit - SDS
• 48800 - Urine DNA Isolation Kit (Slurry Format) - SDS
• 50100 - Urine DNA Isolation Maxi Kit (Slurry Format) - SDS

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FAQs (Selected)

Sample Storage:
Use Norgen’s Urine Preservative for nucleic acid and protein preservation at ambient temperature.

Centrifugation:
Fixed-speed centrifuge may reduce yields; perform all steps at room temperature.

Processing Variable Volumes:
Protocols can be adjusted for 50 μL–2.6 mL urine by scaling reagent volumes.

Frozen Samples:
Thaw gently at room temperature or 37°C for 5 min; do not centrifuge before processing.

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Citations

Recent publications using Norgen’s Urine DNA Isolation Kits include studies in Microbiology Spectrum (2024), Equine Veterinary Journal (2023), Microorganisms (2023), Viruses (2023), mBio (2016), Scientific Reports (2015), and others.

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